Yokogawa Electrical Company’s newest single-cell evaluation answer, the Single Cellome™ System SS2000, has been featured in an article printed within the high-profile journal Analytical Chemistry relating to groundbreaking work performed by researchers on the College of Surrey within the rising subject of single-cell lipidomics. The SS2000 is a dwell cell picture machine geared up with Yokogawa’s authentic dual-microlens spinning disk imaging expertise, and allows cutting-edge life science analysis.
Lipidomics is the large-scale examine of lipids, a big household of molecules that play organic roles starting from sustaining regular operation of the human physique to the event of main ailments. Lipidomic research are conventionally performed by bulk isolation and evaluation of lipids from many cells. Whereas bulk lipid analyses can present scientists a inhabitants common of the lipid profile, they’re unable to discern refined cell-to-cell variations or reveal the spatial or temporal variations to the lipidome brought on by cell-cell interactions.
Single-cell lipidomics is an rising subject the place the lipid composition of single cells is analyzed. These research assist overcome the challenges of bulk lipidomics by providing scientists a method of exploring spatial and temporal variations along with intercellular variability. Understanding these variations is vital to making a extra full image to know ailments similar to most cancers. One problem going through researchers is the power to isolate single cells in a method that maintains the pure lipidome of a cell. Present strategies of single-cell isolation detach and droop a number of cells directly after which isolate them via a slender channel, however this may be significantly hectic to cells and should lead to alterations to mobile lipid make up. Yokogawa’s Single Cellome System SS2000 makes use of confocal imaging expertise to assist abrogate challenges with single-cell lipidomics. It’s a dual-purpose system that allows dwell cell imaging and likewise performs totally automated single-cell and subcellular sampling with out going via the suspension course of, thereby minimizing stress on the cells.
SS2000 utility instance
Yokogawa’s collaborators on the College of Surrey have proven that Yokogawa’s SS2000 is an unlimited enchancment over different single-cell isolation applied sciences within the context of single-cell lipidomics. With the power to pattern adherent cells in tradition, the SS2000 has allowed researchers to take care of the pure lipid profile of sampled cells whereas additionally offering spatial and temporal info based mostly on actual time imaging. The researchers have taken benefit of this distinctive twin functionality to create a workflow that isn’t possible with different applied sciences. They’ve coupled the spatial and temporal info gained from the SS2000’s dual-microlens spinning disk imager with liquid chromatography mass spectrometry information performed on every sampled cell. This novel strategy is a radical step ahead within the subject of lipidomics, which is able to assist result in breakthroughs in various therapies and therapies for most cancers, diabetes, and heart problems.
On this method, Yokogawa will proceed to supply our clients with new worth and the newest expertise, and assist guarantee well-being for all.
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